Inhibitory effects of quinoline isolated from Ruta chalepensis and its structurally related derivatives against α-amylase or α-glucosidase

Abstract

This study was to isolate an active component of the chloroform fraction from the methanol extract of Ruta chalepensis leaves and to measure inhibitory effects against α-glucosidase or α-amylase. The inhibitory compound of R. chalepensis leaves was isolated using chromatographic methods and identified as quinoline. Quinoline and its structurally related derivatives were tested for their inhibitory activities by evaluating the IC50 values against α-amylase or α-glucosidase and were compared with that of acarbose. Based on the IC50 values, quinazoline exhibited the greatest inhibitory activity (20.5 µg/mL), followed by acarbose (66.5 µg/ mL), and quinoline (80.3 µg/mL) against α-glucosidase. In case of α-amylase, quinazoline had potent inhibitory activity, followed by quinoline (179.5 µg/mL) and acarbose (180.6 µg/mL). These results indicate that R. chalepensis extract, quinoline, and quinazoline could be useful for inhibiting α-glucosidase or α-amylase.