A comprehensive in silico analysis of functional and structural impact SNPS in the MC1R gene

Abstract

The aim of this study was to analyze the genetic variation that can alter the expression and the function of the MC1R gene using computational methods. Out of the total 222 SNPs, 22 were found to be non-synonymous (ns) SNPs, 9 were found in the 5' upstream and 7 were found in the 3'UTR. It was found that these 6 nsSNPs rs3212366, rsl 1547464, rsl 805009, rsl 805008 and rsl 805007 were damaging by both the SIFT and the Poly Phen servers. We identified, a mutation from Phe to Leu at positions 196 (rs3212366) on the surface of the protein caused the greatest impact on stability. The rs3212362, rs3212379 and rs35025176 in the 5' upstream were suggested might change the MC1R gene expression levels by FastSNP. Based on the in silico search, the rs3212369 located in the putative Hsa-miR-421 target sequences might have some effect onMClR gene expression. © Medwell Journals, 2011.