Effect of nitrogen limitation on long-side-chain poly-β-hydroxyalkanoate synthesis by Pseudomonas resinovorans

Abstract

Pseudomonas resinovorans produced poly-β-hydroxyalkanoates (PHAs) when grown on hydrocarbons but not on glucose. In a chemostat culture, the PHA composition was β-hydroxybutyrate (C4)-β-hydroxyhexanoate (C6)-β- hydroxyoctanoate (C8)-β-hydroxydecanoate (C10) (1:15:75:9) on octanoate and C4-C6-C8-C10 (8:62:23:7) on hexanoic acid. Contrary to the reported behavior of Pseudomonas oleovorans, the PHA accumulation rate increased under ammonium limitation on octanoate.