Sweet Sorghum: Salt Tolerance and High Biomass Sugar Crop

Abstract

beta-cyclodextrin-coated sensors chips were obtained by grafting amino-beta-cyclodextrin or amino-polymers of beta-cyclodextrin (poly-beta-CD-NH2) to functionalized gold surfaces. An additional carboxymethylated dextran bearing adamantyl groups (Ad-Dex-COOH) was then immobilized onto the surface by formation of inclusion complexes between beta-cyclodextrin cavities and adamantyl groups. Such multilayered structures were stable in aqueous media. However, the initial beta-cyclodextrin-coated surface could be recovered by using sodium dodecylsulfate solutions (SDS). After activation with N-hydoxysuccinimide, dextran-coated sensor chips were used to bind antibodies. The immunoreactivity of the resulting biosensors was examined. Moreover, conditions leading to the complete regeneration of the initial surface were investigated. Throughout this study, interfacial adsorption and desorption phenomena were followed in real time by an optical technique, Surface Plasmon Resonance (SPR).