Effect of Sodium Sulfate on the Hydrolysis of Steroid Glucuronides and Nonsteroid Glucuronides with the β-Glucuronidase Preparations from Bovine Liver, Helix Pomatia and E. Coli

Abstract

The rate of hydrolysis of steroid glucuronides (pregnanediol-, and 17-hydroxycorticosteroid-glucuronides) is increased by an addition of sodium sulfate to the incubation medium, when usinng β-glucuronidase preparations from bovine liver. But, sodium sulfate inhibits the enzyme hydrolysis of pregnanediol-, and estriol-glucuronides with preparations from Helix pomatia and from E. coli. The rate of hdrolysis of nonsteroid glucuronides (P-nitrophenyl-and phenolphthaleinglucuronides) is increased by sodium sulfate when using Preparations from bovine liver and Helix pomatia, it is inhibited with preparations from E. coli. The preparation from Helix pomatia consists of four glucuronidase fractions, none of which hydrolyzes steroid glucuronides specifically. © 1977, Japan Society of Clinical Chemistry. All rights reserved.