The chemical constituents from the roots of Gentiana crassicaulis and their inhibitory effects on inflammatory mediators NO and TNF-α

Abstract

Chemical study on the roots of Gentiana crassicaulis Duthie ex Burk (Gentianaceae) afforded 15 compounds, including two new iridoid glycosides, qinjiaosides B (1) and C (2). Their structures were elucidated by spectroscopic methods and chemical evidence. The isolated iridoid glycosides 1, 4–6 and 8–11 were tested for their anti-inflammatory activity by the inhibitory effects on LPS-induced NO and TNF-α production in macrophage RAW264.7 cells. All of them showed inhibitory effects on inflammatory mediators NO at a concentration of 15 µM, while 5 and 9 displayed the most potential inhibitory effects on TNF-α with IC50 of 0.06 and 0.05 µM, respectively. The structure-activity relationships (SARs) of these iridoid derivatives were discussed. Graphical abstract: [Figure not available: see fulltext.].