A Novel Locally Prepared Inactivated Bivalent Mycoplasma Vaccine for Chicken Flocks in Egypt

Abstract

Mycoplasma is a vertically transmitted disease causing severe economic losses in poultry industry, so this study aimed to prepare a local inactivated a bivalent Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) vaccine. Vaccine was adjuvanted with Montanide ISA70 then evaluated for safety, sterility, and potency. The vaccine efficacy was evaluated in 50 SPF one day old chicks which divided into 5 groups 10 birds each. Chicks were vaccinated with 1st dose at 7 days of age and boostered with the 2nd dose after 3 weeks. Three weeks post vaccination with 2nd dose birds were challenged, chicks were kept under observations for 10 weeks. During the experiment data of clinical signs, daily mortality, body weight, postmortem, serum samples and nasal swabs were collected. serum samples were collected weekly after first vaccination till the third week after challenge and humoral immune response was estimated against Mycoplasma strains using the enzyme-linked immunosorbent assay (ELISA) test and results revealed that vaccines gave a high protective antibody titer, and the vaccine gave 80% protection against MG and 90% against MS. From our result locally prepared inactivated bivalent vaccine gave a promising result in birds protection against serious avian pathogens and considered as an effective tool for the prevention of mycoplasmosis in layers and breeders chicken farms in Egypt.