Green synthesis, characterization and antimicrobial activity of copper nanoparticles derived from Fusarium oxysporum

Abstract

The formation of nanoparticles from microbes has received special attention in wide range of applications like antimicrobial activities, less toxic to environment and many others. The present research uses fungus Fusarium oxysporum for the extracellular synthesis of copper nanoparticles from copper acetate solution. The UV-Vis spectroscopy and change in solution color was used as a confirmation for formation of nanoparticles. The copper nanoparticles characterization was performed by using XRD, FTIR and SEM. FTIR results indicates possible functional groups involved in reduction of copper ions into copper nanoparticles and their stabilization. XRD pattern cleared that the biosynthesized nanoparticles had face-centered cubic (FCC) geometry with crystalline nature. The size and morphology of as-synthesized nanoparticles was determined by using SEM micrographs. Antimicrobial activity against different bacterial strains such as Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus was studied by measuring the zone of inhibition and Minimum inhibitory concentration was also studied.