Analysis of mutant of potato (Solanum tuberosum L.) cultivar Kennebec

Abstract

Potatoes are horticultural commodity that have high economic value. Kennebec is one potato (Solanum tuberosum L.) cultivar suitable for potato chips, therefore it is able be used as genetic resource to develop superior potato cultivars. By gamma ray irradiation at a dose of 15 Gy to cv. Kennebec, one mutant clone, i.e. clone 53.1, had been obtained. This research had an objective to analyse the clone 53.1 as a mutant of potato cv Kennebec. Molecular analysis by RAPD marker using 9 primers namely OPA 1, OPA 2, OPA 5, OPA 7, OPA 8, OPA 9, OPA 10, OPB 8 and OPB 18 showed that the mutations occured at all nine loci and 36.67% alleles had mutated. The highest level of mutation occurred at the OPA 2 locus. Mutations also significantly affected morphological characters in the form of plant height at 45 and 90 days after planting (DAP), tuber diameter, tuber number, tuber weight, shoot weight, root weight, color of tuber eye and color of basal stem of sprouting tuber. The plant height and the tuber productivity of clone 53.1 as a mutant are higher than that of potato cv Kennebec as an original cultivar.