Ion Pair Chromatograpy: A Critical Prespective

Abstract

Reversed phase chromatography is one of most popular modes of chromatography in use. Ion pair chromatography a subset of reverse phase chromatography even though happens due to ionic interactions, needs a reverse phase stationary phase as support. Retention of basic analytes on reverse phase columns is the most commonly encountered situation where ion pair chromatography is used. Reproducibility problems in gradient elution, slow equilibration times are some of the disadvantages of ion pair chromatography for which mixed mode columns can provide the best possible solution. In this concise review, an attempt has been made to give an overview of all aspects of ion pair chromatography.