Effect of novel marine nutraceuticals on IL-1 -mediated TNF- release from UVB-irradiated human melanocyte-derived cells

Abstract

UV-induced inflammation and reactive oxygen species formation are involved in the development of melanoma. Natural products like 5-scymnol and CO2-supercritical fluid extract (CO2-SFE) of mussel oil contain anti-inflammatory and antioxidant properties that may aid in reducing the deleterious effects of UV radiation. Therefore, their effect on the release of the proinflammatory cytokine, tumour necrosis factor- (TNF-), from UVB-irradiated human melanocytic cells was examined. Human epidermal melanocytes (HEM) and MM96L melanoma cells were exposed to UVB radiation and IL-1. Cell viability and TNF- levels were determined 24 hours after-irradiation while p38 mitogen-activated protein kinase (MAPK) activation was observed at 15min after-irradiation. When -tocopherol, CO2-SFE mussel oil, and 5-scymnol were added to the UVB-irradiated HEM cells treated with IL-1, TNF- levels fell by 53, 65, and 76, respectively, while no inhibition was evident in MM96L cells. This effect was not due to inhibition of the intracellular p38 MAPK signalling pathway. These compounds may be useful in preventing inflammation-induced damage to normal melanocytes. © 2011 Visalini Muthusamy et al.