Antibiotic Sensitivity against Proteus Mirabilis Isolated from Pus Samples in Tertiary Care Hospital of Quetta, Pakistan

Abstract

Abstract   Proteus is an important microorganism that have been isolated from wound infections causing severe complications. This study was aimed to isolate and determine the antibiotic sensitivity profile against isolates. A total of 50 wound and pus samples were collected from patients admitted in BMC Hospital, Quetta and processed in Pathobiology Laboratory of Center for Advance Studies of Vaccinology and Biotechnology (CASVAB) University of Baluchistan. All the samples were inoculated onto Brain Heart infusion (BHI), MacConkey agar, Cysteine-Lactose-Electrolyte-Deficien (CLED) and Blood agar and incubated at 37 oC, overnight. The suspected colonies were identified by gram stain and different biochemical tests and also confirmed by local made Quick Test Strip 12 (QTS 12). Among the total 50 samples 8 were found positive for Proteus mirabilis. Antimicrobial susceptibility of Proteus mirabilis against different antibiotic was evaluated by Kirby-Baur Method. Four different antibiotics were tested and ampicillin was found highly susceptible (75%), followed by Gentamicin (50%), Ceftazidime (40%) and ciprofloxacin (40%). This study shows the incidence of Proteus mirabilis in wound infection and best possible antimicrobial therapies against its infection.