Detection of circulating tumor cell subpopulations in patients with head and neck squamous cell carcinoma (HNSCC)

Abstract

Background: Since image based diagnostic tools fail to detect early metastasis in head and neck squamous cell carcinoma (HNSCC) it is crucial to develop minimal invasive diagnostic methods. A promising approach is to identify and characterize circulating tumor cells (CTC) in the peripheral blood of HNSCC patients. In this pilot study, we assessed which non-hematopoietic cell types are identifiable and whether their numbers differ in pre- And postoperative blood samples. Copyright: Methods: 20 ml citrated peripheral blood was taken from 10 HNSCC patients before and after curative resection. CTC were enriched using density gradient centrifugation. CTC presence was verified by multi-immunofluorescence staining against cytokeratin (CK; epithelial), N-cadherin (mesenchymal); CD133 (stem-cell), CD45 (hematopoietic) and DAPI (nucleus). Individual cell type profiles were analyzed. Results: We were able to detect cells with epithelial properties like CK+/Ncadherin2/ CD452 and CK+/CD1332/CD452 as well as cells with mesenchymal features such as N-cadherin+/CK2/CD452 and cells with both characteristics like N-cadherin+/CK+/CD452. We also observed cells showing stem cell-like features like CD133+/CK2/CD452 and cells with both epithelial and stem cell-like features such as CD133+/CK+/CD452. The number of CK positive cells (p50.002), Ncadherin positive cells (p50.002) and CD133 positive cells (p50.01) decreased significantly after resection. Kaplan-Meier test showed that the survival was significantly shorter when N-cadherin+ cells were present after resection (p50.04; 474 vs. 235 days; [HR]53.1). Conclusions: This is - To the best of our knowledge- The first pilot study identifying different CTC populations in peripheral blood of HNSCC patients and showing that these individual cell type profiles may have distinct clinical implications.