Evaluation of Wound Healing Capacity of Selected leaf Extracts using In vitro Scratch Assay with L929 Fibroblas

Abstract

Wound healing is the process by which the skin repairs and maintains itself. Any delay in the healing might result in various skin pathologies like prolonged non-healing and chronic ulceration. Traditional medicines use plant-based products that play an important role in cutaneous wounds. L929 Fibroblast cell line forms the connective tissues, which hold importance by synthesizing extracellular matrix and collagen in the process of wound healing. In the present study, cold percolated ethanol leaf extracts of Beta vulgaris and Psidium guajava were compared for its in vitro wound healing activity through scratch wound assay performed on L929 cells. The rate of healing was examined at regular intervals and determined using ImageJ software. The skin cell re-epithelialization property was identified to be comparatively higher levels in the ethanol extract of Beta vulgaris when compared to that of Psidium guajava. This study aims to compare the in vitro wound healing activity of the selected ethanol extracts with that of the standard positive control, thereby extending its application for in vivo wound healing capacity useful in the cosmetic industries.