Synthesis and phosphatase activity of a Cobalt(II) phenanthroline complex

Abstract

Abstract: A mononuclear cobalt(II) complex, [Co (phen) 2Cl 2], (phen = 1,10-phenanthroline) has been synthesized and structurally characterized by different spectroscopic methods including single crystal X-ray structural study. X-ray crystal structural analysis revealed that the cobalt(II) complex crystallizes in a monoclinic system with C2 / c space group and exists in cis-configuration in its crystalline state. Room temperature magnetic measurement accounts for 3e paramagnetism and indicates high spin cobalt(II) in the solid state. The cobalt(II) complex has been evaluated as a functional model for phosphatase enzyme by using 4-nitrophenylphosphate (PNPP) as a standard substrate in aqueous DMF medium. This mononuclear cobalt(II) complex exhibits good hydrolytic phosphoester cleavage efficiency with kcat value of 3.78 × 10 2h - 1. Graphical Abstract: SYNOPSIS The cobalt(II) complex of phenanthroline exhibits good catalytic activity towards 4-nitrophenylphosphate (PNPP) as a standard substrate in aqueous DMF medium with kcat value of 3.78×102h-1. [Figure not available: see fulltext.].