Genetic characterization of indigenous anatolian water buffalo breed using microsatellite DNA markers

Abstract

One indigenous water buffalo population to Anatolia was characterised with 11 cattle autosomal microsatellite loci. A set of 4 cattle microsatellite loci was found to be polymorphic in the Anatolian buffalo genome. Genotyping of these polymorphic microsatellite loci revealed alleles ranging from 3 to 9. The observed heterozygosity ranged from 0.550 to 0.775 and the expected heterozygosity ranged from 0.494 to 0.815. The FIS value within each locus, changed from -0.101 to 0.205. Total FIS was 0.043 indicating that Anatolian water buffalo population samples seemed to be in Hardy- Weinberg expectation.