Antimicrobial resistance of Aeromonas spp. isolated from the sea trout (salmo trutta L.) in Latvia

Abstract

The aim of this study was to investigate the patterns of antibiotic resistance of Aeromonas spp. bacteria isolated from the sea trout (Salmo trutta) from the state fish hatcheries of the Institute of Food Safety, Animal Health and Environment 'BIOR', Latvia. Bacteriological investigations were performed at four state fish hatcheries located in the drainage basins of the main Latvian rivers-Daugava, Venta and Gauja, during the five-year period (2012-2016). In fish with visible clinical signs, bacteriological samples were collected from heart, liver, spleen, kidney and ulcer surfaces. Aeromonas hydrophila and Aeromonas salmonicida were isolated from sea trouts. A total of 52 individual sea trouts were examined. Resistance to amoxicillin, ampicillin, cephalexin, colistin, doxycycline, enrofloxacin, erythromycin, florfenicol, gentamycin, kanamycin, lincomycin, neomycin, oxytetracycline, spectinomycin, streptomycin, tetracycline, trimethoprim/sulfamethoxazole was tested. The results of this study suggest a multi-drug resistance pattern among the A. hydrophila isolates. All the isolates were resistant to amoxicillin (100%), ampicillin (100%), cephalexin (100%) and erythromycin (100%). The lowest level of resistance was found against florfenicol (4.55%), gentamycin (4.55%), kanamycin (4.55%), but susceptibility was recorded to enrofloxacin, neomycin and trimethoprim/sulfamethoxazole. A. salmonicida isolates were resistant to oxytetracycline (9.38%) and tetracycline (9.38%). For other antibiotics A. salmonicida isolates were susceptible.