Electrophoresis profile of total peroxidases in sera and saliva of patients with different oral tumors

Abstract

Total peroxidase system (EC 1.11.1.X) activity is known to play a key role in a number of human diseases, where the activity of these species can be both beneficial & detrimental. In our previous work (submitted for publication) a remarkable increase have been noticed in the activity of this system in saliva of patients with oral tumors (Oral Squamous Cell Carcinoma OSCC), & Ossifying Fibroma, OF). The present project aimed to highlight the variations in the different forms of this system in saliva & serum samples among patients with above mentioned tumors, in comparison to that of corresponding healthy individuals, using the electrophoresis as the analytical tool. Salivary peroxidase gave faint bands with a poor separation when the analysis was carried out using basic PAGE electrohoresis while good clear bands, as well as better resolutions of these bands were obtained when acidic PAGE electrophoresis was used for the analysis. An additional band, moved further toward the anode, was observed to be present, as the electrozymogram indicated, in the saliva samples of the patients with malignant tumors (Squamous cell carcinoma). The results also showed that using benzidine, or o-dianisidine, as the substrate in staining of the Polyacrylamide gels, in order to localize the bands that exhibit peroxidase activity, seems to be better than using 3,3′,5,5′- Tetramethylbenzidine (TMBZ) as the substrate for this purpose.