Crystallization and preliminary X-ray crystallographic analysis of human myotubularin-related protein 1

Abstract

Myotubularin-related protein 1 is a phosphatase that dephosphorylates phospholipids such as phosphatidylinositol 3-phosphate or phosphatidylinositol 3,5-bisphosphate. In this study, human MTMR1 was overexpressed in Escherichia coli, purified and crystallized at 277K using polyethylene glycol 20000 as a precipitant. Diffraction data were collected to 2.0Å resolution using synchrotron radiation. The crystals belonged to space group P1, with unit-cell parameters a = 67.219, b = 96.587, c = 97.581Å, α = 87.597, β = 86.072, γ = 77.327°. Assuming the presence of four molecules in the asymmetric unit, the calculated Matthews coefficient value was 2.61Å3Da-1 and the corresponding solvent content was 52.9%.