In-vivo Anti-Diabetic Efficacy of Silver Nanoparticles from Marine Brown Seaweed Colpomenia sinuosa on Alloxan Stimulated Hyperglycemic Activity in Wistar Albino Rats

Abstract

Diabetes mellitus is a chronic disease of the endocrine system characterized by elevated blood glucose levels, and disturbances in carbohydrate, fat and, protein metabolism. In the present study, the anti-diabetic efficacy of biosynthesized silver nanoparticles from marine brown seaweed Colpomenia sinuosa has been evaluated. The anti-diabetic effect of the silver nanoparticles biosynthesized from the Colpomenia sinuosa was assessed by inducing diabetes in the experimental Wistar albino rats through Alloxan monohydrate, a chemical that ultimately results in hyperglycemia (increase in the fasting blood sugar level) at a dosage of 50 mg/kg body weight given orally for about 28 days. The outcome of treatment with silver nanoparticles (50 mg/Kg i.p.) biosynthesized from Colpomenia sinuosa, were estimated using various biochemical parameters. The fasting blood glucose levels have reduced in the affected animals to near-normal levels. The retention of the level of the enzymes involved in diabetes, hematological analysis, decrease in the levels of total cholesterol, triglycerides, low-density lipoprotein, and phospholipids in the silver nanoparticles treated animals compared to the levels in normal control animals, exhibited significant anti-diabetic activity as compared to glipizide.