Influence of Silver and Copper Nanoparticles on Physiological Characteristics of Phaseolus vulgaris L. in vitro and in vivo

Abstract

Phaseolus vulgaris seeds were surface sterilized using ethanol 90%, soaked in a sterile distilled water for 60min, then soaked in copper and silver nanoparticles at different concentrations (25, 50 and 100mg/ml) for 15 and 30min. The effect of nanoparticles on the plant physiological characteristics in vivo was detected. Callus was initiated from treated and non–treated seed embryos, then embryos were maintained and transferred to callus induction medium Murashige and Skoog (MS) medium supplemented with combinations of plant growth regulators including Dichlorophynoxyacetic acid (2,4-D) (2.5mg/l), Benzyl adenine (BA) (0.5mg/l). The effects of nanoparticles on %callus induction, callus fresh weight and callus dry weight were observed. Results indicated that the effective concentration of nanoparticles that affect the physiological parameters of treated plant, % callus induction, callus fresh and dry weight was 50mg/ml sliver nanoparticles at 30min.