Detection of extended spectrum beta-lactamase producing Klebsiella pneumoniae and Escherichia coli of environmental surfaces at upper Egypt

Abstract

This study was aimed to evaluate the rate of environmental contamination of Extended- spectrum β-Lactamase (ESBL) and non ESBL-producing1 Klebsiella pneumoniae (K, pneumoniae) and Escherichia coli (E. coli) as well as the susceptibility to 13 antimicrobial agents. Moreover, this study documents two antibiotic resistance genes as well as highlights their important role in spreading ESBL-producing bacteria among environmental surfaces at upper Egypt. A total of 227 environmental isolates, K. pneumoniae (n = 102) and E. coli (n = 125) were recovered from 750 samples of 6 different environmental surfaces at upper Egypt. ESBL production was observed in 80 isolates, for overall prevalence of 56.25% with a predominance of K. pneumoniae (45/80), followed by E. coli prevalence of 43.75% with a predominance of (35/80). The resistance rate was higher among ESBL producers than non ESBL producers. All of K. pneumoniae and E. coli ESBL producers were found resistance (100%) to cephalothin, ampicillin, cefuroxime, cefataxine, ceftriaxone and ceftazidime. The resistance rate (%) was higher in aztreonam (93.3 and 100) and cefotaxime (95.5 and 91.42) then gentamicin (84.4 and 42.8) ciprpfloxacin (77.7 and 68.5) followed by cotrimoxazole (46.6 and 60) of ESBL-producing K and E strains, respectively. BCR analysis demonstrated that 80.3% of ESBL-K and 66.6% of ESBL-E isolates harbored SHV β-lactamase followed by 66.6% of ESBL-K and 0.0% of ESBL-E isolates harbored TEM β-lactamase. © 2013 Academic Journals Inc.