Several pests and diseases affect banana crop in Ecuador and Cucumber mosaic virus (cmv) is one of the most important pathogens. The aim of this research was to standardize a new molecular approach to achieve a sensitive and highly specific detection of cmv in Ecuadorian bananas. Specific primers were designed from the sequence encoding for the virus capsid protein. PC-F1, PC-RD1 and K-F primers, obtained from cDNA replicated from RNA of infected banana, allowed accurate virus detection by Reverse transcription and Hemi-nested PCR. Virus detection was possible even in asymptomatic plants, providing a technology with potential use for the Ecuadorian banana producers.
CITATION STYLE
Buitrón-Bustamante, J. L., & Morillo-Velastegui, L. E. (2017). Estandarización de un método de detección molecular del Cucumber mosaic virus (cmv) en banano ecuatoriano. Corpoica Ciencia y Tecnologia Agropecuaria, 18(1), 113–124. https://doi.org/10.21930/rcta.vol18_num1_art:562
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