Gonad development and blood plasma profile of female bada fish (Rasbora sp.) by giving a combination of the hormones estradiol and spirulina in the feed

Abstract

This study aimed to evaluate the effect of the combination of estradiol-17β hormone and spirulina in feed on blood plasma biochemistry, histology, and gonad development of female bada fish (Rasbora argyrotaenia). The research method were an experimental study using a completely randomized design with four treatment combinations and four replications, namely without the addition of the estradiol-17β hormone and spirulina (P0), the addition of the estradiol-17β hormone at 7 g kg-1 feed (P1), the addition of spirulina at 30 g kg-1 feed (P2) and the addition of the estradiol-17β hormone at 7 g kg-1 feed and spirulina at 30 g kg-1 feed. Female bada fish (4.22±1.28 g) were kept in an aquarium measuring 100x50x50 cm3 with a density of seven individuals per aquarium. Fish were fed the treatment twice daily, with 3% of the biomass for six weeks. The test parameters were estradiol-17β hormone concentration, egg diameter, blood plasma glucose concentration, blood plasma total cholesterol concentration, and gonadal histology observations. The experimental results showed that the concentration of the estradiol-17β hormone in the body plasma of treatments P1 and P3 was statistically significantly different (P<0.05) compared to treatments P0 and P2. The combination of estradiol-17β hormone and spirulina added to the feed gave not significantly different results (P>0.05) on blood plasma biochemistry and egg diameter of bada fish broodstock. A combination of estradiol-17β hormone and spirulina hormones in the feed resulted in better P1 and P3 treatments with higher plasma concentrations of the estradiol-17β hormone. Observations of gonadal development from histology showed that the bada fish gonads belonged to the asynchronous type.