Prevalence of AGER Gene Polymorphism in Post Menopause Iraqi Sample with Osteoporosis and Osteopenia in type 2 Diabetes Mellitus

Abstract

Osteoporosis (OP) is one of the most important metabolic disorders which is affected by interaction of genetic and environmental factors by almost 70% and 30% respectively. Genetic components are identified to strongly effect bone mineral density, bone building and turnover, so they play central role in determining risk of OP and fragility fractures. This case-control study consists of patient and control groups; Group A: (70) postmenopausal women with OP and osteopenia, Group B: (20) control group. five milliliters of blood sample were divided into three tubes; one tube (1ml) contain gel for obtain serum to measure glucose level, the others tubes containing ethylene-diamine-tetra-acetic acid (EDTA), in second tube 2ml stored in deep freeze at (–40 Co) until genomic analysis of DNA for the performance of PCR genotyping of gene polymorphisms of RAGE, in third tube 2ml used to perform Glycated Hemoglobin % (HbA1c) assays. HbA1c and Serum glucose levels is significantly increased in Group A. frequency of C allele and CC genotype of rs1800625 were high significant in group A(p<0.001) than control. A allele and AA genotype of rs1800624 were high significant in group A(p<0.001) than control. Homozygous 1800625 were (31%) and heterozygous 1800625(31%) compared to control homozygous 1800625 (5%) and heterozygous 1800625 (5%) respectively. Homozygous 1800624 were (28.5%) and heterozygous 1800624(28.5%) in group A compared to control homozygous 1800624 (5%) and heterozygous 1800624 (20%) respectively. In conclusion, the CC genotype and C allele of rs1800625 SNP and AA genotype and A allele of rs1800624 SNP can be considered as indicators for OP in post menopause Iraqi women with type2 DM.