Isolation and identification of antibacterial compounds from Vernonia colorata leaves

Abstract

Vernonia colorata (Compositae) is used throughout Africa for a variety of ailments. This prompted the screening of this plant for biological activity. Previous experiments carried out in our laboratory revealed that the leaves possessed high antibacterial activity. Through conventional chromatographic techniques and bioassay-guided fractionation, the following sesquiterpene lactones were isolated and identified by spectroscopic data; vernolide (1), 11β, 13-dihydrovernolide (2) and vernodalin (3). Only 2 is a novel compound, although its antibacterial activity is low compared to compounds 1 and 3 which had minimum inhibitory concentrations of 0.1-0.5 mg/ml against Gram-positive bacteria. © 2002 Elsevier Science Ireland Ltd. All rights reserved.