Linkage analysis of isozyme gene loci in Picea abies (L.) karst

Abstract

In total 14 isozyme gene loci were tested for segregation in Picea abies. Single tree segregation was not significantly heterogeneous. Pooled over all trees, segregation at the Idh-1 and Pgi-2 gene loci deviated significantly from the Mendelian expectation. Out of 91 two-locus combinations 57 could be analysed for linkage. The recombination frequencies were estimated employing the maximum likelihood and the Bayesian likelihood methods. Estimations of the recombination rates confirmed the close linkage group of Got-1: Pgi-2 in Pinaceae. Also Dia-2 appears to be linked to Pgi-2 and Got-1. Moderate linkage groups of Got-2: Skdh-1 and Lap-2: Pgi-2 were found but not in all of the investigated trees. © 1989, The Genetical Society of Great Britain.