Inheritance of salt tolerance in wild soybean (Glycine soja Sieb. and Zucc.) accession PI483463

Abstract

Tolerant soybean (Glycine max [L.] Merr.) cultivars aid in reducing salt damage in problem fields. New genes are important to reduce losses from salt injury. Objectives of this study were to determine inheritance of salt tolerance in wild soybean (Glycine soja Sieb. and Zucc.) PI483463 and to test allelism of tolerance genes from genotypes PI483463 and S-100, a common ancestor of southern in US cultivars. Tolerant (T) PI483463 was crossed to sensitive (S) cultivar Hutcheson to study inheritance. PI483463 (T) was crossed with S-100 (T) to test for allelism. Parents, F 1 plants, F 1 populations, and F 2:3 lines were assayed in a 100 mM salt solution to determine tolerance. F2 from T × S cross segregated 3(T):1 (S) and the F 2:3 lines responded 1 (T): 2 (segregating):1 (S). F 2 plants from PI483463 (T) × S-100 (T) segregated 15 (T):1 (S) indicating different genes from the 2 sources. Results showed that G. soja line PI483463 had a single dominant gene for salt tolerance, which was different than the gene in G. max line S-100. The symbol, Ncl2, was designated for this new salt tolerance allele. © The American Genetic Association. 2009. All rights reserved.