SUMOylation of the nuclear pore complex basket is involved in sensing cellular stresses

Abstract

The nuclear pore complex (NPC) is the major conduit for nucleocytoplasmic transport and serves as a platform for gene regulation and DNA repair. Several nucleoporins undergo ubiquitylation and SUMOylation, and these modifications play an important role in nuclear pore dynamics and plasticity.Here,we perform a detailed analysis of these post-translational modifications of yeast nuclear basket proteins under normal growth conditions aswell as upon cellular stresses,with a focus on SUMOylation.We find that the balance between the dynamics of SUMOylation and deSUMOylation of Nup60 and Nup2 at the NPC differs substantially, particularly in G1 and S phase. While Nup60 is the unique target of genotoxic stress within the nuclear basket that probably belongs to the SUMO-mediated DNA damage response pathway, both Nup2 and Nup60 show a dramatic increase in SUMO ylation upon osmotic stress, with Nup2 SUMOylation being enhanced in Nup60 SUMO-deficientmutant yeast strains. Taken together, our data reveal that there are several levels of crosstalk between nucleoporins, and that the post-translational modifications of the NPC serve in sensing cellular stress signals.