Stability of an Aspirin in the Aspirin+Curcumin Admixture at Different Storage Conditions

Abstract

Aims: The pure stability of aspirin in the aspirin (100 μg/mL) only and aspirin (100 μg/mL) +curcumin (600 μg/mL) admixt. without any ingredient under two solvents (distd. water, DW and normal saline, NS), three storage temps. (25°C, 4°C and -20°C) and periods (1st, 3rd and 7th days) was evaluated. Study Design: The injectable DW- and NS-aspirin contg. solns. in the lab. cap polyethylene bottle were stored and evaluated at controlled temp. (25°C, 4°C and -20°C) during 7 days. Methodol.: Effects of admixt. compds., periods of storage and temp. of storage on the concns. of active compd. (aspirin) were analyzed. The concn. of aspirin in each soln. was detd. by stability-indicating high-performance liq. chromatog. (HPLC)-UV detection. A 1.0 mL vol. of each sample was withdrawn and reconstituted with 3.0 mL of ethanol and directly injected into HPLC system immediately after filtration at 1st, 3rd and 7th days for anal. The stability of the solns. was detd. by calcg. the percentage of the initial aspirin concns. remaining at each test condition and periods. Stability was defined as the retention of at least 90% of the initial aspirin concn. Results: The concn. of aspirin of the aspirin only and aspirin + curcumin admixt. solns. remained at least 90% of original without any color change or pptn. in the DW and NS soln. at 4°C and -20°C throughout 7 day period and showed instability that decreased gradually below 90% of original concns. after 1 day at 25°C in the two solns. Conclusion: Two kinds of solns. of only aspirin and aspirin + curcumin admixt., in DW and NS, showed different stability dependence on temp. of storage that means maintained stability at 4°C and -20°C and did not show effect of admixt. of curcumin on aspirin stability during 7 days except 25°C. [on SciFinder(R)]