Extraction of Useful DNA from Different Parts of the Durian (Durio zibethinus) Fruit

Abstract

Successful deoxyribonucleic acid (DNA) extraction from the durian fruit allows effective molecular identification and genotyping of durian cultivars for quality assurance. While most genetic identification of durian samples used the leaf as the DNA source, studies exploring durian fruit as a source of DNA for molecular identification and genotyping are limited. In this study, four potential sources of genetic material: the peduncle, spine, carpel, and aril from the durian fruit were evaluated for DNA yield (in terms of concentration), quality (in terms of ratio of A260/A280), and suitability in PCR amplification (using genetic markers that target the chloroplast, nuclear, and microsatellite DNA regions). Results of this study show that the spine is a good potential source of genetic material alongside the peduncle.