The potential use of the Trypanosoma cruzi metacyclic trypomastigote (MT) stage-specific molecule glycoprotein-82 (gp82) as a vaccine target has not been fully explored. We show that the opsonization of T. cruzi MT with gp82-specific antibody prior to mucosal challenge significantly reduces parasite infectivity. In addition, we investigated the immune responses as well as the systemic and mucosal protective immunity induced by intranasal CpG-adjuvanted gp82 vaccination. Spleen cells from mice immunized with CpG-gp82 proliferated and secreted IFN-γ in a dose-dependent manner in response to in vitro stimulation with gp82 and parasite lysate. More importantly, these CpG-gp82-immunized mice were significantly protected from a biologically relevant oral parasite challenge.
CITATION STYLE
Eickhoff, C. S., Giddings, O. K., Yoshida, N., & Hoft, D. F. (2010). Immune responses to GP82 provide protection against mucosal Trypanosoma cruzi infection. Memorias Do Instituto Oswaldo Cruz, 105(5), 687–691. https://doi.org/10.1590/S0074-02762010000500015
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