Determination of disk diffusion and MIC quality control guidelines for high-dose cefepime-tazobactam (WCK 4282), a novel antibacterial combination consisting of a β-lactamase inhibitor and a fourth-generation cephalosporin

Abstract

High-dose cefepime-tazobactam (1:1; WCK 4282), a novel antibacterial combination consisting of the β-lactamase inhibitor tazobactam and a fourth-generation cephalosporin, is under clinical development for the treatment of serious Gramnegative infections. A quality control (QC) study was performed to establish disk diffusion and MIC ranges for cefepime-tazobactam for multiple QC reference strains. The cefepime-tazobactam QC ranges for a fixed tazobactam MIC of 8g/ml and disk diffusion (30/20-g disk) test methods were approved by the CLSI Subcommittee on Antimicrobial Susceptibility Testing in January 2015 and January 2016. These QC ranges will be important for accurate in vitro activity evaluations of cefepimetazobactam when tested against clinical Gram-negative bacteria during clinical studies and routine patient care.