Gravitropism of oat and wheat coleoptiles: Dependence on the stimulation angle and involvement of autotropic straightening

Abstract

Gravitropism of oat (Avena sativa L.) and wheat (Triticum aestivum L.) coleoptiles was investigated in relation to the displacement angle or to the initially set stimulation angle (SA). We measured curvature rates at the early phase of curvature, before it was affected by the drop in SA resulting from the curvature response itself. The plot of the rates against the sines of initial SAs revealed similar curves for oats and wheat, which approached saturation as the sine increased to unity. The two species and previously analyzed rice [Iino et at. (1996) Plant Cell Environ. 19: 1160] appeared to have similar gravisensitivities. Initial SAs below and over 90°yielded comparable rates when the sine values were the same, indicating that the extent of gravitropism is determined by the gravity component perpendicular to the organ's long axis. Long-term curvature kinetics at different SAs indicated that the net curvature rate dropped sharply before the tip reached the vertical position and then the tip approached the vertical slowly, with clear oscillatory movements in the case of wheat. During this late curvature phase, the coleoptile straightened gradually, although none of its parts had yet reached the vertical. When rotated on horizontal clinostats or displaced upwards to reduce SA in the late curvature phase, coleoptiles bent in the opposite direction. These results demonstrated that autotropism counteracts gravitropism to straighten coleoptiles.