Effect of genetic variation of TLR-4 gene on immune response of diabetic patients with normal Albuminuria, microalbuminuria and macroalbuminuria in Najaf Governorate, Iraq

Abstract

TLR4 gene polymorphisms are known to impair corresponding signaling pathways in immune and non immune cells followed by weakened adaptive immune responses. This phenomenon can be seen in several inflammatory diseases such as rheumatoid arthritis (RA), cancer, asthma and for coronary artery disease, atherosclerosis and diabetes. A lot of studies indicated that polymorphisms of TLR4 were correlated with immunity-related diseases, such as chronic inflammation and atherosclerosis diseases. The present study was designed to examine TLR-4 gene polymorphisms Asp299Gly (rs4986791) (+1196C/T)and Thr399Ile (rs4986790) (896A/G) effect on some immune markers IL-1β, IL-6, TNF-α, HMGB1,and ICAM-1 in Type 2 Diabetes Militias patients, inspected two single nucleotide polymorphisms (SNPs) of TLR-4 gene. The study included the TT genotype IL-1β concentration were significantly higher in microalbuminuria (31±13.1) than control group (25.9±3.4) which is lower than Macro Albuminuria group (31.2±12.5), TNF-α concentration in CT genotype were higher in Micro Albuminuria and macroalbuminuria (962.9 ±53.3), (965±51.5) groups respectively than normoalbuminuria group (786.9±567.3). Considered to GG genotype HMGB1 concentration were significantly higher in control group (1193.82±189.69) than Macro Albuminuria group (500.46±423.54), AA and AG genotypes of IL-6 plasma concentration were significantly higher in control group (T2DMNormo Albuminuria) (30.49±7.9,26.97±45.49), (19.45±8.5, 20±6.17) respectively than control group (12.42±6.27,13.5±8.67).