Quality control standardization and comparative anthelmintic activity of different extracts from evolvulus alsinoides l

Abstract

The main objective of this investigation was to perform physicochemical and phytochemical standardization and to evaluate the anthelmintic activity of different extracts of the whole plant of Evolvulus alsinoides. Quality control standardization of the whole plant was carried out following the World Health Organization guidelines. Further, 6 different extracts of Evolvulus alsinoides were prepared with n-hexane, petroleum ether, chloroform, acetone, ethanol and water. High performance thin layer chromatography analysis was performed to quantify the content of luteolin in these extracts. In vitro anthelmintic activity was screened on Indian adult earthworm due to its similarities to intestinal roundworms of humans. Doses of 100 mg/ml of each dried extract were mixed with PBS and earthworms were placed in them. Paralysis and/or death of earthworms were taken as a criterion for anthelmintic activity. Most of the physicochemical parameters were found to be within the prescribed limits of World Health Organization guidelines, while, the plant material did not show the presence of any aflatoxins. Ethanol extract of Evolvulus alsinoides showed potent anthelmintic activity and the maximum amount of luteolin was also found to be present in the ethanol extract compared to other extracts. Thus, the potent anthelmintic activity of ethanol extract of Evolvulus alsinoides could possibly be attributed to the presence of luteolin.