Yogurt enriched to correct intestinal microflora in dysbiosis

Abstract

The high incidence of dysbiotic disorders, growing threat of new intestinal bacterial pathogens with multidrug resistance and high virulence, and high level of xenobiotic contamination of food products and the environment require the search for alternative non-medicinal products aimed at improving and maintaining health of the intestinal tract. The authors developed a recipe and technology of two-layer yogurt enriched with a prebiotic complex, containing dietary fiber of wheat bran, indigestible food components that promote the growth of normal intestinal microflora (bifidobacteria and lactobacilli), an inactivated yeast culture of Saccharomycescerevisiae (vini), and blueberry and cornelian cherry puree - a source of polyphenolic compounds. The yoghurt culture used in the technology contains freeze-dehydrated strains of probiotic cultures, i.e., Streptococcus thrmophilus, Lactobacillus delbrueckiissp.bulgaricus, Lactobacillus acidophilus, Bifidobacterium lactis, and lactose. The authors studied the effect of the developed yogurt on the intestinal microflora and their ability to prevent and correct dysbiosis. To this end, a model of experimental antibiotic-associated dysbiosis was reproduced using a broad-spectrum antibiotic Gentamicin in white mice. The antibiotic was being injected intraperitoneally for 5 days. After withdrawal of gentamicin, a considerable increase in opportunistic enterobacteria (Enterobacteraerogenes, Enterobactercloacae, and Citrobacterfreundii), Proteussp, and Candidasp. was observed. There was also a decrease in the quantity and quality of Lactobacillussp. E.coli and a decrease in the content of Bifidobacteriumsp. Enriched yogurt in the diet of mice allowed the microbiological evidences of dysbiosis to be eliminated in a short time, prevented clinical symptoms of dysbiosis, i.e., decreased physical activity and poor appetite, stool softening, constipation, flatulence, and worsening the appearance. Thus, the developed enriched yogurt can be used to prevent and correct dysbiotic intestinal disorders.