Effect of Cichorium intybus L. seed extract on renal parameters in experimentally induced early and late diabetes type 2 in rats

Abstract

Blood and urine biochemistry screening tests are important for initial detection of diabetes, determination of severity of its complications, and monitoring of therapy. We evaluated the effects of aqueous chicory seed extract (CSE), on renal biochemical parameters, histology, and Na+/glucose cotansporters, SGLT1 and SGLT2 expression levels using metformin, and aspirin as controls. Late stage type 2 diabetes (LT2D; FBS,>300mg/dl) and early stage type 2 diabetes (ET2D; FBS, 140-220mg/dl) were induced in rats by streptozotocin (STZ group) and a combination of STZ and niacinamide (NIA/STZ group), respectively. A non-diabetic group was included as control. Treatment included daily intraperitoneal injections of either CSE (125mg/kg b.w.) or metformin (100mg/kg b.w.) and oral aspirin (120mg/kg b.w.) for 21 days. At the end, blood and 24h urine samples were collected; and kidneys were saved at −80 ˚C. CSE reduced urinary α1-microgobulin excretion in ET2D (p=.043), and serum uric acid (p=.045), and glomerular diameter (p