In vivo depletion and genetic targeting of mouse intestinal CX3CR1+ mononuclear phagocytes

Abstract

Mononuclear phagocytes (MPs) are an essential component of the intestinal immune system. They are comprised of a few dendritic cell and macrophage subsets, all with the common ability to sample extracellular milieu and to discriminate between dangerous and innocuous signals. Despite the commonality, each MP subset acquires distinct developmental pathways and unique functions, likely to fulfill needs of the tissue in which they reside. Some MP subsets develop from monocytes and are distinguished by their expression of CX3C-chemokine receptor 1 (CX3CR1). This manuscript summarizes our expertise in vivo targeting of intestinal CX3CR1+ MP subsets. The described tools might be useful for studies of CX3CR1+ MP function in various murine experimental models, particularly under non-inflammatory conditions.