Anti-angiogenic and cytotoxic evaluation of green-synthesized Fe2ZnO4 nanoparticles against MCF-7 cell line

Abstract

The use of plants for nanoparticle (NP) synthesis, grounded in green chemistry principles, is an environmentally friendly and economically viable approach. In the present study, the leaf extract of Elaeagnus angustifolia L. was used as a biosynthetic agent to generate bimetallic zinc oxide NPs. The present study investigated the effect of ZnO NPs on anti-angiogenesis and cell migration. Various bimetallic NPs, including zinc-iron oxide and nickel-zinc oxide, under-went characterization through Fourier-transform infrared spectroscopy and X-ray Diffraction within the 25-65˚ range. Confirmation of NP formation was determined by identifying the surface plasmon resonance peak. MTT assay was used to determine the cytotoxic properties of E. angustifolia L. extracts, ZnO NPs and associated metals in MCF-7 breast cancer cells. The plant extract demonstrated antiproliferative effects at 200 µg/ml, whereas E. ang-Fe2 ZnO4 NPs showed varying cytotoxic effects based on concentration. The rat aortic ring and cell migration assays illuminated anti-angio-genic attributes, with the E. ang-Fe2 ZnO4 NPs blocking blood vessel development entirely at 100 µg/ml, implying profound anti-angiogenic efficacy. Therefore, E. ang-Fe2 ZnO4 NPs may serve a role in antiangiogenic therapy.