Polyisoprenoids profile and composition from selected plant Sapotaceae family

Abstract

This present study reports the polyisoprenoids (polyprenol/dehydrodolichol and dolichol) profile, and composition from selected Sapotaceae leaves and roots namely Manilkara zapota, Ma. kauki, and Mimusops elengi. The existence and circulation of polyisoprenoids were investigated using two-dimensional thin layer chromatography (2D-TLC). The polyisoprenoid profile in the leaves and roots were observed and categorised into two types. Type-I, depicting a dominating of dolichols over polyprenols (100%) was detected in Ma. zapota roots only. These dolichols occurred one longer-chain dolichol family (C75-C105). Type-II, expressing the existence of both polyprenols and dolichols, was detected in the leaves of Ma. zapota, Ma. kauki, and Mi. elengi. Similarly, type-II was also found in the roots of Ma. kauki and Mi. elengi. Dolichol concentrations and relative proportions were more abundances found than polyprenols both in the leaves and roots. Polyprenols in the leaves and roots were characterised chain length of C75-C120. By contrast, dolichols occurred shorter and longer-chains in the leaves of Ma. kauki (C55-C140 and more) and Mi. elengi (C45-C140 and more). The present study suggested that the pattern of medium-chain polyprenols, longer-chain polyprenol, shorter dolichols, and longer dolichols occurred in Sapotaceae family.