Biosynthetic study of amphidinolide W

Abstract

The biosynthetic origins of amphidinolide W (1) were investigated on the basis of 13C-NMR data of 13C-enriched samples obtained by feeding experiments with [1-13C], [2-13C], and [1,2- 13C2] sodium acetate in cultures of a strain Y-42 of the dinoflagellate Amphidinium sp. These incorporation patterns suggested that 1 was generated from a hexaketide chain, two acetate units, four isolated C 1 units from C-2 of acetates, and four branched C1 units from C-2 of acetates. The acetate-incorporation patterns for C-1-C-2-(C-21) and C-8-C-18-(C-23, C-24) of 1 corresponded well to those for C-1-C-2-(C-27) and C-5-C-15-(C-28, C-29) of amphidinolide H (2) isolated from this strain. © 2002 Pharmaceutical Society of Japan.