USP7: Combining tools towards selectivity

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Abstract

Active-site directed probes based on the ubiquitin scaffold have been successfully applied as tools to determine the levels of active deubiquitinating enzymes (DUBs). Here, we show the development of a Ub-based reagent selective for the DUB USP7. This concept can be applied for the generation of other new selective reagents.

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Gjonaj, L., Sapmaz, A., González-Prieto, R., Vertegaal, A. C. O., Flierman, D., & Ovaa, H. (2019). USP7: Combining tools towards selectivity. Chemical Communications, 55(35), 5075–5078. https://doi.org/10.1039/c9cc00969h

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