Preventive effects of an original combination of grape seed polyphenols with amine fluoride on dental biofilm formation and oxidative damage by oral bacteria

Abstract

Aims: To investigate the preventive effects of an original combination of a grape seed extract (GSE) with an amine fluoride (Fluorinol®) on dental plaque formation and oxidative damage caused by oral bacteria. Methods and Results: The antibacterial activity of the compounds was assessed using the broth macrodilution method, and their antiplaque activity was evaluated on a multispecies biofilm grown on saliva-coated hydroxyapatite discs. The effect on glucosyltransferases activity was analysed through reductions in the overall reaction and the quantity of insoluble glucan synthesized. The combination of 2000 μg ml-1 of GSE with 10·2 mg ml-1 of Fluorinol® significantly decreased the biofilm formation (up to 4·76 log10 of reduction) and inhibited by 97·4% the insoluble glucan synthesis by glucosyltransferases. The antioxidant activity of this combination, alone or incorporated into a formulated mouthwash (Eludril daily®), was determined using the Trolox equivalent antioxidant capacity assay (TEAC), and both showed significantly greater antioxidant capacity than vitamin C. Conclusions: The GSE/Fluorinol® combination showed both a significant antiplaque activity and an important antioxidant capacity in vitro, without any bactericidal effects. Significance and Impact of the Study: This is, to our knowledge, the first report on the properties of an original combination of a polyphenolic extract with amine fluoride that could be used for the prevention of oral diseases and oxidative damage associated. © 2013 The Society for Applied Microbiology.