Rosemary leaf extracts were obtained by supercritical fluid extraction (SFE) and Soxhlet extraction. Their chemical compositions were evaluated by GC-MS. The extracts were analyzed for compounds reported in the literature as showing antimicrobial and antioxidant activities. The rosemary extracts were tested with regard to antioxidant (DPPH radical scavenging and total phenolic content - Folin-Denis reagent), antibacterial (Gram-positive bacteria - Staphylococcus aureus ATCC 25923 and Bacillus cereus ATCC 11778 - and Gram-negative bacteria - Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853) and antifungal (Candida albicans) activities. Antioxidant, antibacterial and antifungal activities of the SFE extracts were confirmed.
Genena, A. K., Hense, H., Smânia, A., & De Souza, S. M. (2008). Rosemary (Rosmarinus officinalis) - A study of the composition, antioxidant and antimicrobial activities of extracts obtained with supercritical carbon dioxide. Ciencia e Tecnologia de Alimentos, 28(2), 463–469. https://doi.org/10.1590/S0101-20612008000200030