Isolation and genomics DNA amplification of Kapur (Dryobalanops sumatrensis) from North Sumatra

Abstract

Kamper is one of high value tree species from North Sumatra. Its timber has high economic value and has been targeted for exploitation since decades. Identification of species diversity and further their origin and the evolutionary process is important in the conservation strategy of the targeted species. The aims of this research were to get the information about optimal isolation of DNA genomics procedure and primer amplification for Kamper species by applying various primer tested, those were: ITS 2, matK Kim 1, trnH-psbA, trnL-trnF (c and d), trnL-trnF (c and f) and rbcL. The DNA extraction was conducted by CTAB method with some mild modifications to a few stages. The result showed that the modified CTAB method could yield good quality DNA. rbcL with the primer volume as much F: 1μl and R: 1 μl produces the best-amplified band.