The quality of fresh and frozen semen Bali bull assessed from different ejaculate volume

Abstract

Semen volume is the amount of ejaculate released by male Bali cattle during one ejaculation, which consists of spermatozoa and seminal plasma. The purpose of this study was to determine the effect of Bali cattle ejaculation volume on the quality of fresh and frozen semen. Semen collection was carried out using an artificial vaginal collection method. The collected semen was then evaluated with microscopic parameters: motility, viability, and abnormalities. Semen came from 7 male Bali bulls. The data obtained were analyzed by one-way ANOVA. The results showed that there was no significant difference (P>0.05) between the volume of Bali cattle ejaculation on the quality of fresh semen and also post thawing based on the parameters tested. The sperm concentration of Bali bulls has average 935.75 ± 80.25 x 106. The average percentage of fresh semen motility was 70.79±1.26 while that for frozen was 47.83±1.89. The average viability of fresh spermatozoa was 80.04±3.68 while frozen 52.87±0.67. The average abnormality value of fresh spermatozoa was 8.54±1.88 while frozen was 23.83±4.22. Based on these results, it was concluded that the different volume of ejaculate of Bali cattle did not affect the quality of fresh semen of Bali cattle, both fresh and frozen based on the parameters of motility, viability, and abnormalities.