The effect of liposomal epigallocatechin gallate and metoclopramide hydrochloride co-administration on experimental migraine

Abstract

Epigallocatechin gallate (EGCG) has been intensively studied for its anti-oxidative, anti-inflammatory and anti-nociceptive effects. Our study aimed to assess the beneficial effect of liposomal EGCG (L-EGCG) co-administered with metoclopramide (MC) on oxidative stress and pain in experimental migraine induced by i.p. nitroglycerine (NG) administration in rats. Five groups of randomly divided rats (7/group) were investigated: control (C, group 1) with i.p. administration of saline solution, NG control group (group 2), NG+MC (group 3), NG+MC+EGCG group (group 4), and NG+MC+L-EGCG (group 5). The nociception was appreciated by the formalin test and the oxidative stress/anti-oxidant status by serum tests. MC alone significantly improved the nociception process and the oxidative stress parameters but not the antioxidative status. Adding EGCG to MC significantly reduced the oxidative stress and antioxidant status together with decreasing of nociception, with better results for L-EGCG.