Toxicity and compound identification of padinaaustralis extract

Abstract

People awareness of the importance of health has increased significantly in the last decade, it forces people to find alternative treatments which are cheaper and safer when compared with the use of synthetic drugs. Research on antibacterial activity of Padina australis against Escherichia coli have been conducted, but it does not identified yet which active compounds in the Padina australis extract that has potential as an antibacterial. Identification phytol compounds from the extracts of Padina australis and toxicity tests have been conducted using BSLT and GCMS methods. The results of this research identify that Padina australis extract with ethanol has antibacterial activity value of LC50 177.83 ppm. Phytochemical test results show that active ingredient of Padina australis extract is terpenoid compounds, and that triterpenoids has potential as an antibacterial. GC-MS test result shows that active compound of Padina australis extract consists of phytol compound which has 90-99% similarity with steroids, phenols, fatty acids, carboxylic acids, hydrocarbons, and proteins. Compound identification test of active Padina australis extracts through GC-MS methods show that phytol compounds are useful as antibacterial and the toxicity test results show that phytol compounds are not cytotoxic.