Comparative phytochemical and antioxidative characterization of trifolium pratense L. And ocimum basilicum L.

Abstract

The phytochemical composition of Trifolium pratense L. and Ocimum basilicum L. was investigated by using chromatographic and spectrophotometric methods. T. pratense extract was rich in ferulic acid (237.48mg/100g dry weight) and chlorogenic acid (158.50 mg/100g dry weight), meanwhile O. basilicum extract presented the highest concentration in ferulic acid (437.58 mg/100g dry weight). The determination of total phenolic and flavonoid contents indicated that O. basilicum contained the highest amounts (67.89mg GAE/100g dry extract and 6.49 mg QE/mL extract respectively), aspect which was corelated with its strongest antioxidant activity assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl), FRAP (ferric reducing antioxidant power) and CUPRAC (cupric reducing antioxidant capacity) methods.